Pluto Workstation

Automated Antibody Cocktailing and Cell Staining in One Station

The Pluto Workstation automates the full flow cytometry sample prep workflow—antibody cocktailing, cell staining, and wash steps—with an SOP-like interface and proprietary centrifuge-free, pipette-based washing. Designed for labs running increasingly complex panels, Pluto standardizes sample prep across users and sites, reducing variability that manual workflows introduce.  

Used by pharma, flow cores, and CROs, Pluto supports high-parameter assays, reduces total pipetting steps, and enables consistent, repeatable runs for high-throughput processing.  

Get more information

What the Pluto Workstation Does 

End-to-end automation for flow cytometry sample prep 

  • Antibody cocktail generation 
  • Plate-based cell staining 
  • Gentle C-FREE™ wash (centrifuge-free washing) 
  • Mixing, incubation, and reagent additions 
  • Walkaway operation 
  • High-throughput processing 

Where it fits 

  • Flow cores needing consistency across dozens of users 
  • Labs building or transferring complex high-parameter panels 
  • Pharma groups needing standardization across sites 
  • Automation teams integrating reproducible upstream prep with spectral or conventional analyzers 

 

Why Labs Choose Pluto 

  1. Saves hands-on time with one-station automation
    By combining cocktailing, staining, and C-FREE washing in a single platform, Pluto removes manual transfers and pipetting steps—freeing researchers from hands-on prep and eliminating workflow interruption. 
  2. Easy UI and fast setup
    A guided, SOP-style interface simplifies method setup, minimizes training time, and reduces hesitation around adopting automation. 
  3. Enhance reproducibility
    Pluto standardizes high-parameter antibody cocktailing and staining, reducing manual errors for consistent results across users and prep days. 
  4. Familiar labware compatibility
    Supports a variety of commercial antibody vials, Curiox standard 96-well plates, deep-well plates, tubes, and commonly used pipette tips—making Pluto easy to adopt within existing lab setups. 
  5. Minimal assay optimization
    Pluto supports most manual sample prep protocols with little to no adjustment, enabling straightforward transition to automated workflows. 
  6. Enhanced traceability
    Visual and digital tracking tools record reagent volumes, antibody usage, and liquid-handling history to improve reproducibility and support compliance needs. 

 

Powered by C-FREE™ 

Pluto replaces centrifuge washes with precision-controlled liquid handling 

Pluto uses our proprietary C-FREE™ technology to perform centrifuge-free washes. Using precise pipette handling, cells remain undisturbed while the liquid handler replaces supernatant. This eliminates the spin-force of centrifuges and the workflow interruptions caused by taking plates off the system for spinning. 

Learn more about C-FREE technology. 

 

Additional Features 

  • Liquid-level detection for accurate aspiration and reduced error handling 
  • Wide 1–1000 µL pipetting range 
  • Broad antibody-vial compatibility  
  • Low reagent waste 
  • Automate FMO and single-color control setup 
  • 21 CFR Part 11 Compliance 
  • Up to 96- or 384-well throughputs 
  • Accommodates high-parameter panels of up to 90 antibodies 

 

Testimonials 

“We got essentially equivalent, sometimes better stain index with the Pluto.” – Holden Maecker, PhD Stanford University 

“We replaced the standard centrifugation step with Curiox technology. Pluto can allow you to basically simulate centrifuge-free cell washing using a standard liquid handler. It’s really a nice technology.” – Raffaello Cimbro, PhD AstraZeneca 

“I had over 200 individual pipette actions that occurred over 4.5 hours. Two of those hours I had to be physically in the lab. Even during the five-minute spins, I couldn’t step away to check email or do anything else.” – Ryan Duggan, AbbVie 

 

Available Configurations  

  • Pluto LT: Single-tip varispan and 8-channel pipette heads  
  • Pluto MT: Single-tip varispan and 24-channel pipette heads  
  • Pluto HT: Four-tip varispan and 96- or 384-channel pipette heads 
     

Custom Options: 

  • Gripper module  
  • Temperature control module  
  • On-board tilter module  

For more details or to customize your instrument, contact us 

Part Number Description Quantity Quantity
CF-PLU-LH08-01 C-FREE, Pluto LT Workstation, 8-channel Liquid Handling Head 1 Request a Quote
CF-PLU-LH08MT-01 C-FREE, Pluto MT Workstation, 3×8-channel Liquid Handling Head 1 Request a Quote
CF-PLU-LH08HT-01 C-FREE, Pluto HT Workstation 1 Request a Quote
Part Number Description Quantity Ordering
CF-PLU-GRT-01 C-FREE, Pluto, Gripper/Tilter Pack 1 Request a Quote
CF-PLU-GR-01 C-FREE, Pluto, Gripper 1 View Product
CF-PLU-TLT-01 C-FREE, Pluto, Tilter 1 Request a Quote
CF-PLU-TMP-01 C-FREE, Pluto, Temperature Module 3 Request a Quote
CF-PLU-FTK-01 C-FREE, Pluto, Fluidic Test Kit 1 Request a Quote
CF-PLU-BCR-01 C-FREE, Pluto, Barcode Reader 1 Request a Quote

 

Part Number Description Quantity Ordering
CF-PLU-TIPS-250 C-FREE, Pipette Tips, 250 µL, 20 Racks 1,920 Request a Quote
CF-PLU-TIPS-1000 C-FREE, Pipette Tips, 1,000 µL, 20 Racks 1,920 Request a Quote
CF-PLU-DPUPLT-25 C-FREE, Pluto, 96-well U-Bottom Deep Well Plate, 2 mL (5 sleeves) 25 Request a Quote
CF-PLU-DPVPLT-25 C-FREE, Pluto, 96-well V-Bottom Deep Well Plate, 2 mL (5 sleeves) 25 Request a Quote
CF-PLU-UPLT-50 C-FREE, Pluto, 96-well U-Bottom Plate, 300 uL (5 sleeves) 50 Request a Quote

 

Part Number Description Quantity Ordering
CF-PLU-INS-01 C-FREE, Pluto, Installation & Calibration 1 Request a Quote
CF-PLU-SY-01 C-FREE, Pluto, 1-year Service and PM 1 Request a Quote
CF-PLU-1PM-01 C-FREE, Pluto, One-time Preventative Maintenance 1 Request a Quote
CF-PLU-TRVL-01 C-FREE, Pluto, Flat Rate Travel 1 Request a Quote

Description

What the Pluto Workstation Does 

End-to-end automation for flow cytometry sample prep 

  • Antibody cocktail generation 
  • Plate-based cell staining 
  • Gentle C-FREE™ wash (centrifuge-free washing) 
  • Mixing, incubation, and reagent additions 
  • Walkaway operation 
  • High-throughput processing 

Where it fits 

  • Flow cores needing consistency across dozens of users 
  • Labs building or transferring complex high-parameter panels 
  • Pharma groups needing standardization across sites 
  • Automation teams integrating reproducible upstream prep with spectral or conventional analyzers 

 

Why Labs Choose Pluto 

  1. Saves hands-on time with one-station automation
    By combining cocktailing, staining, and C-FREE washing in a single platform, Pluto removes manual transfers and pipetting steps—freeing researchers from hands-on prep and eliminating workflow interruption. 
  2. Easy UI and fast setup
    A guided, SOP-style interface simplifies method setup, minimizes training time, and reduces hesitation around adopting automation. 
  3. Enhance reproducibility
    Pluto standardizes high-parameter antibody cocktailing and staining, reducing manual errors for consistent results across users and prep days. 
  4. Familiar labware compatibility
    Supports a variety of commercial antibody vials, Curiox standard 96-well plates, deep-well plates, tubes, and commonly used pipette tips—making Pluto easy to adopt within existing lab setups. 
  5. Minimal assay optimization
    Pluto supports most manual sample prep protocols with little to no adjustment, enabling straightforward transition to automated workflows. 
  6. Enhanced traceability
    Visual and digital tracking tools record reagent volumes, antibody usage, and liquid-handling history to improve reproducibility and support compliance needs. 

 

Powered by C-FREE™ 

Pluto replaces centrifuge washes with precision-controlled liquid handling 

Pluto uses our proprietary C-FREE™ technology to perform centrifuge-free washes. Using precise pipette handling, cells remain undisturbed while the liquid handler replaces supernatant. This eliminates the spin-force of centrifuges and the workflow interruptions caused by taking plates off the system for spinning. 

Learn more about C-FREE technology. 

 

Additional Features 

  • Liquid-level detection for accurate aspiration and reduced error handling 
  • Wide 1–1000 µL pipetting range 
  • Broad antibody-vial compatibility  
  • Low reagent waste 
  • Automate FMO and single-color control setup 
  • 21 CFR Part 11 Compliance 
  • Up to 96- or 384-well throughputs 
  • Accommodates high-parameter panels of up to 90 antibodies 

 

Testimonials 

“We got essentially equivalent, sometimes better stain index with the Pluto.” – Holden Maecker, PhD Stanford University 

“We replaced the standard centrifugation step with Curiox technology. Pluto can allow you to basically simulate centrifuge-free cell washing using a standard liquid handler. It’s really a nice technology.” – Raffaello Cimbro, PhD AstraZeneca 

“I had over 200 individual pipette actions that occurred over 4.5 hours. Two of those hours I had to be physically in the lab. Even during the five-minute spins, I couldn’t step away to check email or do anything else.” – Ryan Duggan, AbbVie 

 

Available Configurations  

  • Pluto LT: Single-tip varispan and 8-channel pipette heads  
  • Pluto MT: Single-tip varispan and 24-channel pipette heads  
  • Pluto HT: Four-tip varispan and 96- or 384-channel pipette heads 
     

Custom Options: 

  • Gripper module  
  • Temperature control module  
  • On-board tilter module  

For more details or to customize your instrument, contact us 

Brochure—Pluto System

Download

Specification Sheet—Pluto LT System

Download

Specification Sheet—Pluto MT System

Download

Specification Sheet—Pluto HT System

Download

User Manual—Pluto LT

Download

User Manual—Pluto MT

Download

Poster—An Automated, Centrifuge-Less Workflow for Cell Staining and Washing of Flow and Mass Cytometry Samples

Download

Poster—Advancing Flow Cytometry Diagnostics

Download

Poster—Evaluating the Curiox C-FREE Pluto System for Whole Blood Immunophenotyping Sample Processing

Download

Poster—A Centrifuge-Less Approach with Pluto System for Automated Whole Blood Sample Preparation

Download

Additional information

Dimensions (D x W x H) - Closed

Pluto LT System:
740 mm x 790 mm x 895 mm
29.1 in. x 31.1 in. x 35.2 in.

Pluto MT System:
740 mm x 790 mm x 895 mm
29.1 in. x 31.1 in. x 35.2 in.

Pluto HT System:
740 mm x 1090 mm x 1085 mm
42.9 in. x 29.1 in. x 42.7 in.

Weight

Pluto LT System:
140 kg
309 lbs

Pluto MT System:
140 kg
309 lbs

Pluto HT System:
215 kg
474 lbs

Dimensions (D x W x H) - Open

Pluto LT System:
740 mm x 790 mm x 1000 mm
29.1 in. x 31.1 in. x 39.4 in.

Pluto MT System:
740 mm x 790 mm x 1000 mm
29.1 in. x 31.1 in. x 39.4 in.

Pluto HT System:
740 mm x 1090 mm x 1168 mm
42.9 in. x 29.1 in. x 46.0 in.

You may also like…

  • Pluto ALPHA

    Pluto ALPHA

  • Pluto Code

    Pluto Code

  • C-FREE Gripper

    C-FREE Gripper