Laminar wash™ Auto 1000 system
Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation.
The Laminar Wash AUTO 1000 System eliminates time-consuming and labor-intensive manual pipetting and handling by automating the complete surface and intracellular staining workflow for flow and mass cytometry. You get all the benefits of laminar washing in a completely automated system.
Laminar Washing Technology
Gentle cell washing in a 96-well format
Optimized Deck Layout
A completely contained system for washer maintenance plates, washer load tray, heater/shaker, cooling device, reagent/buffer plates, input/output stacks and tips.
Laminar Wash AUTO Software
The user-friendly software interface allows sample-specific modifications to methods and comes with pre-programmed protocols to ensure user consistency.
What the AUTO 1000 delivers:
Technology Overview
See the Laminar Wash AUTO 1000 in action.
ACHIEVE TRULY HIGH THROUGHPUT SUSPENSION CELL SAMPLE PREPARATION
The Laminar Wash AUTO 1000 is a completely hands-off automated platform to dispense, fix/permeabilize, stain, wash, and transfer samples for flow and mass cytometry.
“The Laminar Wash’s performance allows us to get very tight control over populations with very tight CV’s largely due to the fact of the clear separations between populations. The maintained ability to detect rare populations is important as we detect the next round of cell therapy products.”
Arnaud Colantonio, PhD.
Associate Director of Analytical Development at AdiCet Bio,
now with Kite Pharma
HOW OUR LAMINAR FLOW TECHNOLOGY WORKS
Compared to a traditional, centrifuge-driven cell washing workflow, laminar cell washing is simpler and faster. With the AUTO 1000, you can prepare 96 samples in just 3 minutes.
AN INNOVATIVE DESIGN DELIVERS BETTER DATA
The power of Curiox’s laminar cell washing technology lies in the unique design of the sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.
Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.
Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.
See how dye is washed away from green fluorescent polystyrene beads during 2 minutes of 7 aspiration and dispense wash cycles. At the end of the cycle, the dye is gone but the polystyrene beads remain in the spot.
Scientific Data
Automated laminar washing technology delivers good consistency and increased retention of low input T cell numbers from freshly thawed PBMC
When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.
Simple and straightforward reproducibility with Laminar Wash Automation
Complement-dependent cytotoxic crossmatch (CDCXM) and flow cytometric crossmatch (FXCM) are both common methods to predict donor-recipient compatibility – the potential success of an organ transplant. FCXM assays are more widely applied across human leukocyte antigen (HLA) labs. FXCM utilizes the use of multiple washing steps, serum treatment and secondary antibody used. Results between the centrifugation-based and Laminar Washing methods within 8 replicates and 3 test sera and 2 controls show average CVs of 12.4% vs 7.9% respectively. With Laminar Wash technology, greater consistency was seen on the Median Channel Fluorescence values gated on B-cells. The ratio of positive crossmatch yielded a higher shift while maintaining a lower MCF value for the negative background. This is attributed by a superior wash and accuracy in matrix serum handling with primary patient cells.
Featured Applications
The Laminar Wash system has been successfully demonstrated on various applications including immunophenotyping and CyTOF.
Flow Cytometry
Gently wash cells without centrifugal stress, allowing higher cell retention with less debris for acquisition and cell sorting.
CELL and Gene Therapy
Improve reproducibility between operators, time, and locations while reducing hands on time for critical process development & CMC activities.
Tumor Microenvironment
Recovery of TILs can be challenging from disocciated tumor samples. Enhance cell retention, improve reproducibility and reduce debris, background, artifacts and clogging.
Technical Specifications
Voltage Requirements |
100-240V |
Power Consumption |
+42V/5.0A |
Dimensions |
89 cm H x 71 CM W x 136 cm D |
Weight |
120kg (264.5lbs) |
Accessories and Components
Laminar Wash™ AUTO1000 Station Standard Configuration |
AUTO-1000-96-01 |
Laminar Wash™ 96-well plate (with a regular lid) |
96-DC-CL-05 |
HEPA Filter |
AU-HE-01-01 |
Laminar Wash™ 96 well grid (LVA) |
DC-GR-96-05 |
Related Products and Accessories
Laminar Wash HT2000
A reliable and easy-to-use benchtop instrument for high throughput flow cytometry labs.
Laminar Wash Mini
An affordable benchtop instrument for low-throughput sample preparation for flow cytometry